 |
Semi-confocal images using a cooled CCD camera
In conventional fluorescence microscopy the inside structure of thick specimens cannot be resolved clearly because of pronounced contribution of out of focus light from above and below the focal plane. It is possible to reject this out of focus light by placing a rotating slit disc in a confocal plane of the microscope. The result is a clear, continous, optically cross-sectioned image that can be recorded with high speed using a cooled CCD camera. The semi-confocal effect alleviates efforts for post image processing.
|
|
 |
Advanced system performance
3D image stacks can be acquired easily using the inverted IX81 or the upright BX61 microscope platforms with their built-in, precise Z motor. Integrated control of DSU, motorised microscope and cooled CCD camera is implemented in Olympus image acquisition software packages, resulting in a complete imaging system solution.
|
|
 |
Easy operation
A simple switch close to the operator's hand makes it easy to control such functions as inserting the slit disc into or removing it out of the light path, exchanging cubes and opening/closing the shutter. It is quick and easy to switch between general fluorescence observation and semi-confocal observation.
|
|
.jpg) |
The right disk for each condition
Five disks are available of varying slit width and spacing allowing the DSU to be optimised for varying objective numerical apertures and specimen thicknesses. Semi-confocal images can be collected with objectives ranging from 10-100x. |
|
 |
Excellent cost efficiency
The DSU is a cost efficient alternative to laser based confocal microscope systems. The arc lamp illumination source (xenon or mercury lamp) present on any fluorescence microscope can be used without modification. Fluorescence excitation characteristics can be flexibly changed according to the users needs by the addition of new filters and dichromatic mirror sets.
|
|
 |
UV fluorescence excitation capable
DSU optical design is optimised for 350-700nm excitation allowing excellent performance from near UV to near IR. DSU accepts standard Olympus filter cubes for excellent system flexibility. The motorised DSU filter turret has six positions and can accept up to 5 different fluorescence filter cubes for excellent system flexibility. IX2 microcope frames can optionally be equipped with a second motorised turret (IX2-FRFACA) |
|
Resolution enhancement by removal
