Applications for FV1000

Acousto-optical tunable filter (AOTF)
A filtering device employed to modulate the wavelength and amplitude of illuminating laser light in the latest generation of confocal microscopes. The AOTF utilises sound waves to modulate the wavelength or intensity of ...

Cameleon
The protein Calmodulin is a ubiquitous intracellular calcium sensor that responds to changes in calcium concentration. Each Calmodulin molecule has four binding sites for Ca2+. The molecule undergoes a conformational change after calcium- binding.

Chimeric proteins
The construction of chimeric proteins is an elegant approach for FRET analysis in living cells (e.g. Cameleon or chimeric peptides for substrate-enzyme reactions). A chimeric protein useful for FRET analysis usually is a molecule, which is ...

Confocal Laser Scanning Microscopy
A popular mode of optical microscopy in which a focused laser beam is scanned laterally along the x and y axes of a specimen in a raster pattern. The emitted fluorescence (reflected light signal) is sensed by a photomultiplier tube and ...

Fluorescence Correlation Spectroscopy = FCS
Fluorescence correlation spectroscopy allows investigating molecular dynamics in highly diluted sample solutions. The researcher yields ...

Fluorescence Lifetime Imaging = FLIM
Multi-colour staining with fluorescent dyes is actively used for observing the distribution of biological materials (such as proteins, lipids, ...

Fluorescent probes
Many fluorescent dyes or fluorochromes can specifically interact with certain target molecules, like most nucleic acid stains (DAPI, ...

Multiphoton Fluorescence Microscopy
Multiphoton fluorescence microscopy is a powerful research tool that combines the advanced optical techniques of laser scanning microscopy with ...

Overlaying Transmission and Fluorescence Images
Often it is interesting to merge fluorescence and transmission images into one image because both sources have different information contents. Such an overlay ...

Spectral unmixing
A major problem in live cell imaging arises from the use of different fluorochromes with overlapping spectra within one sample, impairing a ...

Time Lapse Imaging (in Life Science)
Dynamic processes such as cell growth, metabolic transport and signal transduction are monitored routinely nowadays. The duration of such processes may vary from ...

Yellow Fluorescent Protein (=YFP)
The yellow fluorescent protein: a spectral variant of the green fluorescent protein (see GFP.)

Z-sectioning and three-dimensional Imaging
Microscopy is basically a two-dimensional observation technique while biological samples are three-dimensional, of course. In order to map the entire volume, the specimen can be ...