Specifications for FV1000MPE |
 |
 Optimal for in vivo observation
Deep tissue penetration
Minimised photodamage/phototoxicity
Reduced photobleaching |
 Back to main page |
 Print |
Basic System
Optimised for in vivo imaging, this system allows deeper sections of specimens to be viewed using the multiphoton laser. The optics are designed to have the beam enter the pupil of the objective efficiently. Precise adjustment of the light intensity is possible through use of a highly durable λ/2 Plate & Glan-Thompson prism. The system features a streamlined, compact design to save valuable laboratory space.
| Single photon laser unit |
Visible light laser |
• LD laser: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635mW, 20mW, Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe(G) laser (543nm, 1mW)
• Modulation: Continuously adjustable via an AOTF (0.1 - 100% in 0.1% increments)
• Operating mode: Allows laser turn-off during the retrace period, adjustment of laser power for a given region, and selection of the laser and selection of the laser wavelength |
| AOTF laser combiner |
• Visible light laser platform with implemented AOTF system, ultra-fast intensity with individual laser lines, additional shutter control
• Connected to scanner via single-mode fibre
• Equipped with laser feedback mechanism to limit changes in laser light intensity over time |
| Multiphoton laser unit |
Multiphoton near-infrared pulsed laser |
• For the adjustable wavelengths of a mode-locked Ti:sapphire laser (femtosecond), refer to additional lasers that can be used on page 6: Mai Tai (Spectra-Physics) or Chameleon (COHERENT)
• Can be directly connected to a scanner via Olympus' optics integrating a multiphoton near-infrared pulsed laser
• Multiphoton near-infrared pulsed laser power unit, water-cooled circulating chiller |
| Optics integrated |
• Integrates a multiphoton near-infrared pulsed laser in the scanning unit
• Laser safety measures implemented via a protective cover for laser safety |
| Shutter/attenuator |
λ/2 Plate & Glan-Thompson prism |
| Scanning unit |
Basic configuration |
• Detector: 3 channels for fluorescence detection (photomultipliers), 1 channel for transmitted light detection (photomultiplier)
• Laser ports: visible-light laser port (integrated by fibre), multiphoton near-infrared pulsed laser port (directly integrated)
• Dichromatic mirrors for excitation, dichromatic mirrors for multi photon excitation, dichromatic mirrors for fluorescence,
emission filter, infrared cut filter: using a high-performance filter
• A filter or spectral type of fluorescence detector can be selected
Spectral type: Channels 1 and 2 provided with independent grating and slit
Selectable wavelength range: 1 - 100 nm, wavelength resolution: 2 nm, wavelength switching speed: 100 nm/ms |
| Scanning method |
Light deflection via 2 galvanometer scanning mirrors |
| Scanning modes |
• Pixel size: 64 x 64 - 4096 x 4096 pixels
• Scanning speed: (pixel time): 2 µs - 5ms
• High-speed scanning mode: 16 frames/sec (256 x 256)
• Dimensions: Time, Z, (wavelength) (or any combination thereof)
• Line scan: straight line (includes rotation), free line, point XY scan |
| Photo detection method |
Analogue integration |
| Pinhole |
Single motorised pinhole, adjustable in 0.5 µm increments |
| Field Number |
18 |
| Zoom size |
1 - 50x (adjustable in 0.1x increments) |
| External detector |
External detector: photomultiplier (2 channels) |
| SIM scanner unit |
For single photon laser (optional) |
| Vibration isolation table for a multiphoton near-infrared pulsed laser |
Table size |
1,800(W) x 1,200 (D) mm |
| Microscopes |
Motorised microscopes |
BX61WI |
| System control |
|
• OS: Windows XP Professional (English version)
• CPU: Pentium IV 3.2GHz or larger
• Memory: 2.0GB or larger
• Hard disk: 120GB or larger
• Dedicated I/F board: built-in PC
• Graphics board: Open GL-compliant
• Recording media: Equipped with DVD dual drive
• Monitor: two SXGA 1280 x 1024 19-inch monitors |
| Software |
FV10-ASW |
| Required installation environment |
Room temperature: 20°C — 25°C ± 1°C, humidity: 60% or less, dust level: Class 10000, |
| Power source environment |
|
AC100-120/220-240V 60VA For details, refer to supplied environmental specifications |
AOM System
The AOM module allows high-speed ON/OFF powering of the laser. Local laser radiation of regions of interest is possible, so the FV1000MPE can be used in experiments requiring laser light stimulation, such as photoconversion. Negative chirp comes standard, so efficient multiphoton excitation can be achieved with relatively weak excitation light.
| Single photon laser unit |
Visible light laser |
• LD laser: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635mW, 20mW, Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe(G) laser (543nm, 1mW)
• Modulation: Continuously adjustable via an AOTF (0.1 - 100% in 0.1% increments)
• Operating mode: Allows laser turn-off during the retrace period, adjustment of laser power for a given region, and selection of the laser and selection of the laser wavelength |
| AOTF laser combiner |
• Visible light laser platform with implemented AOTF system, ultra-fast intensity with individual laser lines, additional shutter control
• Connected to scanner via single-mode fibre
• Equipped with laser feedback mechanism to limit changes in laser light intensity over time |
| Multiphoton laser unit |
Multiphoton near-infrared pulsed laser |
• For the adjustable wavelengths of a mode-locked Ti:sapphire laser (femtosecond), refer to additional lasers that can be used on page 6: Mai Tai (Spectra-Physics) or Chameleon (COHERENT)
• Can be directly connected to a scanner via Olympus' optics integrating a multiphoton near-infrared pulsed laser
• Multiphoton near-infrared pulsed laser power unit, water-cooled circulating chiller |
| Optics integrated |
• Integrates a multi photon near-infrared pulsed laser in the scanning unit
• Laser safety measures implemented via a protective cover for laser safety |
| Shutter/attenuator |
AOM |
| Scanning unit |
Basic configuration |
• Detector: 3 channels for fluorescence detection (photomultipliers), 1 channel for transmitted light detection (photomultiplier)
• Laser ports: visible-light laser port (integrated by fibre), multiphoton near-infrared pulsed laser port (directly integrated)
• Dichromatic mirrors for excitation, dichromatic mirrors for multi photon excitation, dichromatic mirrors for fluorescence,
emission filter, infrared cut filter: using a high-performance filter
• A filter or spectral type of fluorescence detector can be selected
Spectral type: Channels 1 and 2 provided with independent grating and slit
Selectable wavelength range: 1 - 100 nm, wavelength resolution: 2 nm, wavelength switching speed: 100 nm/ms |
| Scanning method |
Light deflection via 2 galvanometer scanning mirrors |
| Scanning modes |
• Pixel size: 64 x 64 - 4096 x 4096 pixels
• Scanning speed: (pixel time): 2 µs - 5ms
• High-speed scanning mode: 16 frames/sec (256 x 256)
• Dimensions: Time, Z, (wavelength) (or any combination thereof)
• Line scan: straight line (includes rotation), free line, point XY scan |
| Photo detection method |
Analogue integration |
| Pinhole |
Single motorised pinhole, adjustable in 0.5 µm increments |
| Field Number |
18 |
| Zoom size |
1 - 50x (adjustable in 0.1x increments) |
| External detector |
External detector: photomultiplier (2 channels) |
| SIM scanner unit |
For single photon laser (optional) |
| Vibration isolation table for a multiphoton near-infrared pulsed laser |
Table size |
2,400(W) x 1,500 (D) mm |
| Microscopes |
Motorised microscopes |
BX61WI/ IX81 |
| System control |
|
• OS: Windows XP Professional (English version)
• CPU: Pentium IV 3.2GHz or larger
• Memory: 2.0GB or larger
• Hard disk: 120GB or larger
• Dedicated I/F board: built-in PC
• Graphics board: Open GL-compliant
• Recording media: Equipped with DVD dual drive
• Monitor: two SXGA 1280 x 1024 19-inch monitors |
| Software |
FV10-ASW |
| Required installation environment |
Room temperature: 20°C — 25°C ± 1°C, humidity: 60% or less, dust level: Class 10000, |
| Power source environment |
|
AC100-120/220-240V 60VA For details, refer to supplied environmental specifications |
MPE-SIM System
Multiphoton lasers have been integrated in the imaging unit as well as in the scanner for laser light stimulation. With multiphoton excitation, excitation occurs only in the focal plane, so there is minimal bleaching. Thus, laser light stimulation of a specific site can be performed in a localised manner, in three dimensions. Additionally, this set of optics is equipped with two AOM modules, and exact laser light stimulation of the targeted area may be achieved in 2 dimensions. (Use of the single photon laser for laser light stimulation by attaching it to the dedicated scanner for laser light stimulation is not possible while imaging with the multiphoton laser.)
| Single photon laser unit |
Visible light laser |
• LD laser: 405nm: 50mW, 440nm: 25mW, 473nm: 15mW, 559nm: 15mW, 635mW, 20mW, Multi Ar laser (457nm, 488nm, 515nm, Total 30mW), HeNe(G) laser (543nm, 1mW)
• Modulation: Continuously adjustable via an AOTF (0.1 - 100% in 0.1% increments)
• Operating mode: Allows laser turn-off during the retrace period, adjustment of laser power for a given region, and selection of the laser and selection of the laser wavelength |
| AOTF laser combiner |
• Visible light laser platform with implemented AOTF system, ultra-fast intensity with individual laser lines, additional shutter control
• Connected to scanner via single-mode fibre
• Equipped with laser feedback mechanism to limit changes in laser light intensity over time |
| Multiphoton laser unit |
Multiphoton near-infrared pulsed laser |
• For the adjustable wavelengths of a mode-locked Ti:sapphire laser (femtosecond), refer to additional lasers that can be used on page 6: Mai Tai (Spectra-Physics) or Chameleon (COHERENT)
• Can be directly connected to a scanner via Olympus' optics integrating a multiphoton near-infrared pulsed laser
• Multiphoton near-infrared pulsed laser power unit, water-cooled circulating chiller |
| Optics integrated |
• Integrates a multiphoton near-infrared pulsed laser in the scanning unit
• Laser safety measures implemented via a protective cover for laser safety |
| Shutter/attenuator |
AOM x 2 |
| Scanning unit |
Basic configuration |
• Detector: 3 channels for fluorescence detection (photomultipliers), 1 channel for transmitted light detection (photomultiplier)
• Laser ports: visible-light laser port (integrated by fibre), multiphoton near-infrared pulsed laser port (directly integrated)
• Dichromatic mirrors for excitation, dichromatic mirrors for multiphoton excitation, dichromatic mirrors for fluorescence,
emission filter, infrared cut filter: using a high-performance filter
• A filter or spectral type of fluorescence detector can be selected
Spectral type: Channels 1 and 2 provided with independent grating and slit
Selectable wavelength range: 1 - 100 nm, wavelength resolution: 2 nm, wavelength switching speed: 100 nm/ms |
| Scanning method |
Light deflection via 2 galvanometer scanning mirrors |
| Scanning modes |
• Pixel size: 64 x 64 - 4096 x 4096 pixels
• Scanning speed: (pixel time): 2 µs - 5ms
• High-speed scanning mode: 16 frames/sec (256 x 256)
• Dimensions: Time, Z, (wavelength) (or any combination thereof)
• Line scan: straight line (includes rotation), free line, point XY scan |
| Photo detection method |
Analogue integration |
| Pinhole |
Single motorised pinhole, adjustable in 0.5 µm increments |
| Field Number |
18 |
| Zoom size |
1 - 50x (adjustable in 0.1x increments) |
| External detector |
External detector: photomultiplier (2 channels) |
| SIM scanner unit |
For multiphoton laser |
| Vibration isolation table for a multiphoton near-infrared pulsed laser |
Table size |
3,000 (W) x 1,500 (D) mm |
| Microscopes |
Motorised microscopes |
BX61WI |
| System control |
|
• OS: Windows XP Professional (English version)
• CPU: Pentium IV 3.2GHz or larger
• Memory: 2.0GB or larger
• Hard disk: 120GB or larger
• Dedicated I/F board: built-in PC
• Graphics board: Open GL-compliant
• Recording media: Equipped with DVD dual drive
• Monitor: two SXGA 1280 x 1024 19-inch monitors |
| Software |
FV10-ASW |
| Required installation environment |
Room temperature: 20°C — 25°C ± 1°C, humidity: 60% or less, dust level: Class 10000, |
| Power source environment |
|
AC100-120/220-240V 60VA For details, refer to supplied environmental specifications | The use of lasers with SUB-PICOSECOND pulses for two-photon microscopy is protected by US Pat No. USP5034613, JP Pat No. JP2848952B2, EU Pat No. EP500717B2, EU Pat No. EP807814B1. This technology is under a license from Carl Zeiss MicroImaging GmbH and Cornell Research Foundation Inc.
|
|
|
