| Name |
|
Description |
| Laser source |
Visible |
Select from the following lasers to be mounted on laser combiner:
Multi-line Argon laser (457nm, 488nm, 515nm), total 30mW
HeNe-Green laser (543nm), 1,5mW
HeNe-Red laser (633nm), 10mW |
| |
Violet / UV |
Select from the following:
405nm Laser Diode, 20mW
440nm Laser Diode, 5,3mW
UV-Argon (351nm), 50mW |
| |
AOTF laser combiner |
AOTF intensity control and shuttering for each laser line in UV and visible light range.
Integrated system control for diode lasers. Both controls provide laser turn-off during retrace period (Fly Back Beam Blanking) and REX modescanning. REX (region of excitation) mode permitts laser intensity and wavelength selection for each designated region of excitation. Integrated shutters are included for all lines. |
| |
ND Laser combiner |
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| Scanning Unit |
Standard configuration |
3 internal PMT detectors for fluorescence detection + 1 external PMT detector for transmitted light, 3 laser ports (visible, UV, IR)
Additional optional light paths available, including:
4th Channel PMT detector unit
Fiber port for fluorescence output to fiber optic
SIM Scanner |
| |
Scanning method |
2 galvano scanning mirrors |
| |
Field number (N.A.) |
18 |
| |
Pinhole |
Single motorised pinhole (common for all channels):
Pinhole diameter: 50-800µm (50-300µm for spectral system)
Pinhole continuously adjustable in 0.5µm increments |
| |
Image channel |
Standard 3 confocal channels (3 Photomultiplier detectors) Additional optional output port light path available for optional units 6 position beamsplitter turrets with CH1 and CH2 equipped with independent grating and slit for fast and flexible spectral detection Selectable wavelength bandwith 1-100nm Wavelength resolution 2nm Wavelength switching speed 100nm/sec CH3 with 6 position barrier filter wheel |
| |
Filter selection |
Ion deposition filters. 6 filter positions available for each excitation, spectral and emission filter turret. Automated filter selection through software |
| |
Scanning modes |
Pixel size: 64x64 - 4096x4096
Pixel Dwell time: 2 to 200µsec with unidirectional scan
0,5 or 1µsec with fast bidirectional scan
Normal, Clip, Zoom-In, Bi-Directional Fast Scan, Line, Point
|
| |
Image depth resolution |
12-bit (=4096 gray levels) per channel |
| |
Optical Zoom |
1x - 50x in 0.5x increment ((1x - 10x in 0.1 increment) |
| |
Z-drive |
Integrated motorised focus module of the microscope, minimum increment 10nm |
| |
PMT Detection |
High-sensitivity, side-on PMT detectors, selected for high efficiency.
Analog accumulation (AAC) and hybrid photon counting (HPCM) modes.
Excellent for quantitation of photometric analysis. High S/N for imaging low intensity samples. |
| |
Spectral System (optional) |
Two detection channels, each equipped with an independent diffraction grating and slit for spectral separation and high-speed bandwidth selection.
Selectable wavelength range: 1-100nm per channel
Wavelength change speed: 1ms/100nm |
| Microscopes |
Upright |
BX61, BX61WI |
| |
Inverted |
IX81 |
| External transmitted light unit |
Transmitted light illumination and detection unit |
Module with integrated external transmitted light photomultiplier detector and 100W halogen lamp, motorised switching , fiber adaptation to microscope frame |
| Fluorescence illumination unit |
|
External fluorescence light source with motorised shutter, fiber adaptation to optical port of scan unit. Motorised switching between LSM light path and and fluorescence illumination |
| PC with system control boards |
|
Latest AT compatibel PC configuration Win XP operating system |
| FluoView application Software |
Image acquisition |
Region designation: point, line, free line, clip, clip zoom
2-dimension: XY,XZ,XT and Xl*
3-dimension: XYZ, XYT, XZT, XYl*, XlT* and XlZ*
4-dimension: XYZT, XlZT* and XYlT*
5-dimension: XYlZT*
Real-Time image calculation: Kalman filtering
Time series controller, protocol processor
*Spectral system only |
| |
Image Format |
XML format, JPEG, BMP, TIFF and AVI movie format
8-bit and 16-bit gray scale
24-bit, 32-bit and 48-bit full colour |
| |
Hardware control |
Laser, scanning unit, microscope |
| |
Image display |
Each image display: Single-channel side by side, merge, cropping, tiling, series (Z/t/Lambda) pass and continuous LUT: individual colour setting, pseudo-colour overlay: Lines, text, scale bar, etc. |
| |
Image processing |
Individual filter: Average, Low-pass, High-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement etc. Calculations: Inter-image, mathematical and logical, DIC black ground leveling |
| |
Image analysis |
Overview of fluorescence intensity within an area, histogram, periment measurement for user assigned area, time-lapse measurement, etc. TIEMPO (Time course software) |
| |
3D visualisation |
Interactive volume rendering Free orientation of cross section display 3D animation, left/right stereo pairs, red/green stereoscopic images and cross section, 3D and 2D sequential operation function |
| |
Spectral unmixing |
Fluorescence seperation through spectroscopy: normal and blind modes
Spectral system only |
| |
Statistical Processing |
2D data histogram display, co-localisation |
| |
Others |
Graphic based help, time course software, trigger IN/OUT function, 3D software, offline review station software (optional), XY motorised stage control software (optional) |
| Power Consumption |
|
Microscope: 6Amps
Scan Unit: 6.2Amps
Computer: 4.5Amps
Multi-line Argon laser: 100Volts, 10Amps
HeNe laser: 0.5Amps each |
|
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| Upgrade Kit for FLIM and FCS |
|
The excitation subsystem consists of a pulsed diode laser driver and different laser heads with pulses in the picosecond time scale. The available wavelengths range from 375 nm to 900 nm. For Time and Space Correlated Single Photon Counting (TSCSPC) different types of fast photomultiplier assemblies (PMA) are available. Single photon avalanche diodes (SPADs) can be used for an increased sensitivity, necessary e.g. for single molecule studies or Fluorescence Correlation Spectroscopy (FCS). |
Unmatched 2nm wavelength resolution
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