| Name |
|
Description |
| Laser source |
Visible |
Select from the following lasers to be mounted on laser combiner:
Multi-line Argon laser (457nm, 488nm, 515nm), total 30mW
Ar laser (488nm), 10mW
HeNe-Green laser (543nm), 1,5mW
HeNe-Red laser (633nm), 10mW |
| |
Violet / UV |
Select from the following:
405nm Laser Diode, 6mW
440nm Laser Diode, 0,7mW |
| |
AOTF laser combiner |
AOTF intensity control and shuttering for each laser line in UV and visible light range.
Integrated system control for diode lasers. Both controls provide laser turn-off during retrace period (Fly Back Beam Blanking) and REX mode scanning. REX (region of excitation) mode permitts laser intensity selection for each designated region of excitation. Integrated shutters are included for all lines. |
| |
ND Laser combiner |
Each laser light path is equipped with a variable neutral density Continuously variable (0.1% - 100%, 1% increment) |
| Scanning Unit |
Standard configuration |
2 internal PMT detectors for fluorescence detection + 1 external PMT detector for transmitted light, 2 laser ports (visible, UV / IR)
|
| |
Scanning method |
2 galvano scanning mirrors |
| |
Field number (N.A.) |
20 |
| |
Pinhole |
5-position pinhole |
| |
Image channel |
Selectable from 2-channel (fluorescence) or 2-channel fluorescence) + 1-channle (transmitted light) |
| |
Filter selection |
Manual selection |
| |
Scanning modes |
Pixel size: 256x256 - 2048x2048
Pixel Dwell time: 2 to 8µsec with unidirectional scan
1µsec with fast bidirectional scan
Normal, Clip, Zoom-In, Bi-directional Fast Scan, Line, Point
|
| |
Image depth resolution |
12-bit (=4096 gray levels) per channel |
| |
Optical Zoom |
1X-10X (0.5x step) |
| |
Z-drive |
Step motor / Minimum step 10nm (BX61, BX61WI and IX81combination), 25nm (other microscope combination) |
| |
PMT Detection |
High-sensitivity, side-on PMT detectors |
| |
Spectral System (optional) |
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| Microscopes |
Upright |
BX51, BX61, BX51WI, BX61WI |
| |
Inverted |
IX81FVSF, IX71FVSF (side port) / special laser safe frame |
| External transmitted light unit |
Transmitted light illumination and detection unit |
Module with integrated external transmitted light photomultiplier detector and 100W halogen lamp, motorised switching , fiber adaptation to microscope frame |
| Fluorescence illumination unit |
|
Connect to external mercury light source and microscope via fibre cable |
| PC with system control boards |
|
Latest AT compatibel PC configuration Win XP / 2000 / NT operating system |
| FluoView application Software |
Image acquisition |
Region designation: point, line, free line, clip, clip zoom
2-dimension: XY,XZ and XT
3-dimension: XYZ, XYT and XZT
4-dimension: XYZT
Real-Time image calculation: Kalman filtering, Peak detection
Protocol processor |
| |
Image Format |
multi-dimensional TIFF, JPEG, BMP, TIFF and AVI movie format
8-bit and 16-bit gray scale
24-bit full colour |
| |
Hardware control |
Laser, scanning unit, microscope |
| |
Image display |
Each image display: Single-channel side by side, merge, cropping, tiling, series (Z/t) pass and continuous LUT: individual colour setting, pseudo-colour overlay: Lines, text, scale bar, etc. |
| |
Image processing |
Individual filter: Average, Low-pass, High-pass, Sobel, Median, Prewitt, 2D Laplacian, edge enhancement etc. Calculations: Inter-image, mathematical and logical, DIC black ground leveling |
| |
Image analysis |
Overview of fluorescence intensity within an area, histogram, periment measurement for user assigned area, time-lapse measurement, etc. TIEMPO (Time course software) |
| |
3D visualisation |
3D animation, left / right stereo pairs, red / green stereoscopic images and cross section |
| |
Spectral unmixing |
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| |
Statistical Processing |
2D data histogram display, co-localisation |
| |
Others |
Graphic based help, time course software (optional), trigger IN/OUT function (optional), 3D software / LSM viewer (optional) offline review station software (optional), XY motorized stage control software |
| Power Consumption |
|
Microscope: 6Amps
Scan Unit: 6.2Amps
Computer: 4.5Amps
Multi-line Argon laser: 100Volts, 10Amps
HeNe laser: 0.5Amps each |
For individual researchers or groups
